- Metabolite
- HMDB0001873 (Isobutyric acid)
- Biospecimen
- Feces
- Status
- Detected and Quantified
- Data source
- Referenced
- Concentration
- 80-2260 nmol/g wet feces
- Age
- Adult (>18 years old)
- Sex
- Both
- Condition
- Normal
Comments
Wet feces samples (n=6) were used in the study. Instrument: LC-MS. Technology specification: targeted RP-UPLC-MS/MS (Sciex Qtrap 4000) in negative ionization mode; derivatization with 3-nitrophenylhydrazine (3-NPH). The original concentration was reported as 0.08-2.26 umol/g.
References
- Han J, Lin K, Sequeira C, Borchers CH: An isotope-labeled chemical derivatization method for the quantitation of short-chain fatty acids in human feces by liquid chromatography-tandem mass spectrometry. Anal Chim Acta. 2015 Jan 7;854:86-94. doi: 10.1016/j.aca.2014.11.015. Epub 2014 Nov 15. [PubMed:25479871 ]